ZM-447439
产品说明书
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同义名 : | - |
| CAS号 : | 331771-20-1 | |
| 货号 : | A107524 | |
| 分子式 : | C29H31N5O4 | |
| 纯度 : | 99%+ | |
| 分子量 : | 513.59 | |
| MDL号 : | MFCD08703125 | |
| 存储条件： |
Pure form Sealed in dry, store in freezer, under -20°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
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| 靶点 |
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| 描述 | Cells treated with ZM-447439 enter interphase, proceed normally into mitosis, and assemble bipolar spindles. However, chromosome alignment, segregation, and cytokinesis all fail. ZM-447439 inhibits cell division and the mitotic phosphorylation of histone H3. It prevents chromosome alignment and separation, impairs spindle checkpoint function, and inhibits the kinetochore localization of BubR1, Mad2, and Cenp-E[1]. The inhibition of Aurora kinases by ZM-447439 reduces the phosphorylation of Ser10 on histone H3 in Hep2 cancer cells. Cells treated with ZM-447439 that are arrested in G2/M phase induce multipolar spindles and accumulate 4N/8N DNA, similar to cells with genetic suppression of Aurora-B. Treatment with ZM-447439 can induce apoptosis. The inhibition of Aurora kinases by ZM-447439 is closely associated with a reduction in the phosphorylation of Akt Ser473 and its substrates GSK3α/β Ser21 and Ser9[2]. | ||
| 细胞研究 | |||||
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| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活性说明 | 数据源 |
| human A172 cell | Growth inhibition assay | Inhibition of human A172 cell growth in a cell viability assay, IC50=0.98411 μM | SANGER | ||
| human A498 cell | Growth inhibition assay | Inhibition of human A498 cell growth in a cell viability assay, IC50=2.3669 μM | SANGER | ||
| human ABC-1 cell | Growth inhibition assay | Inhibition of human ABC-1 cell growth in a cell viability assay, IC50=1.40352 μM | SANGER | ||
| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
1.95mL 0.39mL 0.19mL |
9.74mL 1.95mL 0.97mL |
19.47mL 3.89mL 1.95mL |
| 参考文献 |
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