同义名 :	-
	CAS号 :	1808714-73-9
	货号 :	A1060682
	分子式 :	C19H14F2N4O2
	纯度 :	99%+
	分子量 :	368.34
	MDL号 :	MFCD31665468
	存储条件：	Pure form Sealed in dry, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	-
	动物实验配方：

生物活性
描述	The vacuolar H+-ATPase (v-ATPase) is a universal component of eukaryotic organisms, which is present in both intracellular compartments and the plasma membrane. Its proton-pumping action creates the low intravacuolar pH, benefiting many processes such as, membrane trafficking, protein degradation, renal acidification, bone resorption, and tumor metastasis [1]. EN6 is a small-molecule activator of autophagy that covalently targets cysteine 277 in the ATP6V1A subunit of the lysosomal v-ATPase, which activates mechanistic target of rapamycin complex 1 (mTORC1) via the Rag guanosine triphosphatases. EN6-mediated ATP6V1A modification decouples the v-ATPase from the Rags, leading to inhibition of mTORC1 signaling, increased lysosomal acidification and activation of autophagy. EN6 clears TDP-43 aggregates, a causative agent in frontotemporal dementia, in a lysosome-dependent manner [2].
作用机制	EN6 covalently binds to cysteine 277 in the ATP6V1A subunit of v-ATPase .

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	2.71mL 0.54mL 0.27mL	13.57mL 2.71mL 1.36mL	27.15mL 5.43mL 2.71mL

参考文献

[1]Xiao YT, Xiang LX, Shao JZ. Vacuolar H(+)-ATPase. Int J Biochem Cell Biol. 2008;40(10):2002-6. doi: 10.1016/j.biocel.2007.08.006. Epub 2007 Aug 22. PMID: 17897871. [2]Chung CY, Shin HR, Berdan CA, Ford B, Ward CC, Olzmann JA, Zoncu R, Nomura DK. Covalent targeting of the vacuolar H+-ATPase activates autophagy via mTORC1 inhibition. Nat Chem Biol. 2019 Aug;15(8):776-785. doi: 10.1038/s41589-019-0308-4. Epub 2019 Jul 8. PMID: 31285595; PMCID: PMC6641988.