Apcin
产品说明书
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同义名 : | - |
| CAS号 : | 300815-04-7 | |
| 货号 : | A1012823 | |
| 分子式 : | C13H14Cl3N7O4 | |
| 纯度 : | 98%+ | |
| 分子量 : | 438.65 | |
| MDL号 : | MFCD00525727 | |
| 存储条件： |
Pure form Keep in dark place, sealed in dry, room temperature In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : | - | |
| 动物实验配方： |
| 生物活性 | |||
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| 靶点 |
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| 描述 | Apcin, a ligand for Cdc20, is a potent, competitive inhibitor of APC/C(Cdc20) Apcin competitively inhibits APC/C-dependent ubiquitination by binding to Cdc20 and preventing substrate recognition. Apcin occupies the D-box binding pocket flanking the WD40 structural domain, which prolongs mitosis[1][2][3].At concentrations of 25-50 μM for 48 hours, Apcin in combination with proTAME (6, 12 μM) significantly increases MM apoptosis[3].At 25 μM concentration, acting for 2-14 hours, Apcin induces slippage at a slower rate[2].In the concentration range of 50-200 μM, Apcin most effectively stabilises cycB1-NT and securin, with a slightly weaker effect on full-length cyclin B1[1].In the concentration range of 1.5-200 μM for 18 hours, Apcin acts synergistically with proTAME, a cell-permeable TAME prodrug, to prolong the mitotic duration of RPE1 cells[1]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.28mL 0.46mL 0.23mL |
11.40mL 2.28mL 1.14mL |
22.80mL 4.56mL 2.28mL |
