16-(S)-ML188 is a non-covalent SARS-CoV 3CLpro inhibitor with good enzyme and antiviral inhibitory activity.

Dieckol exhibited SARS-CoV 3CL(pro) trans/cis-cleavage inhibitory effects with IC50 values of 2.7 and 68.1 μM, respectively. It formed extremely strong hydrogen bonds to the catalytic dyad (Cys145 and His41) of the SARS-CoV 3CL(pro).

Theaflavin-3,3'-digallate is a tea extract. Theaflavin-3,3'-digallate inhibited 3CLpro with IC50 value of 9.5μM in a fluorogenic substrate peptide assay.

16-(R)-ML188 is a non-covalent SARS-CoV 3CLpro inhibitor with good enzyme and antiviral inhibitory activity.

CE-5 blocks MERS-CoV 3CLpro activity with EC50 value of ∼12.5 μM. It inhibited SARS 3CLpro activity with IC50 value of 30nM and exhibited antiviral effect with EC50 value of 6.9μM.

Tanshinone IIA is a naturally occuring cardioprotective agent which can inhibit NF-κB and AP-1 DNA binding and display antioxidant and anti-inflammatory properties. Tanshinone IIA showed inhibitory effect on PL(pro) with IC50 value of 1.6μM and less inhibitory effect on 3CLpro with IC50 value of 89.1μM.

Zanamivir is the first of two registered neuraminidase inhibitors for the treatment and prophylaxis of influenza. Zanamivir could inhibit SARS-CoV-2 3CLpro main protease.

Tanshinone IIA sulfonate sodium is a water-soluble derivative of tanshinone IIA, which acts as an inhibitor of store-operated Ca2+entry (SOCE), and is used to treat cardiovascular disorders.

Remdesivir is an nucleotide-analog antiviral prodrug. Remdesivir could inhibit SARS-CoV-2 3CLpro main protease.

Tanshinone I, an active principle isolated from the roots of Salvia miltiorrhiza (Danshen), is an inhibitor of type IIA human recombinant sPLA2 and rabbit recombinant cPLA2 with IC50s of 11 μM and 82 μM, respectively, is structurally similar to tanshinone IIA and may possess similar cytotoxic effects on tumor cells. Tanshinone I showed inhibitory effect on PL(pro) with IC50 value of 8.8μM and less inhibitory effect on 3CLpro with IC50 value of 38.7μM.

Saquinavir mesylate is an HIV Protease inhibitor used in antiretroviral therapy. Saquinavir could inhibit SARS-CoV-2 3CLpro main protease.

Hesperetin is a flavanone extracted from the peel of citrus that exhibits inhibition of PDE4 and UGT. Hesperetin has antioxidative and anti-inflammatory effects as well. Hesperetin dose-dependently inhibited cleavage activity of the 3CLpro with IC50 value of 8.3μM in the cell-based assay.

GC376 is a 3CLpro (3C-like protease) inhibitor that shows promise in treating cats with certain presentations of FIP and has opened the door to targeted antiviral drug therapy.

Rhoifolin, a natural product isolated and purified from the leaves of Turpinia arguya Seem, hows monolithic proliferation inhibitory activities against Hela, A549 and MCF-7 cell lines, and may be beneficial for diabetic complications through their enhanced adiponectin secretion, tyrosine phosphorylation of insulin receptor-β and GLUT4. It was found to efficiently block the enzymatic activity of SARS-CoV 3CLpro.

Dihydrotanshinone I, a natural product isolated and purified from the roots of Salvia miltiorrhiza Bge., exhibits dose-dependent inhibition towards HLM-catalyzed propofol glucuronidation. Dihydrotanshinone I showed inhibitory effect on PL(pro) with IC50 value of 4.9μM and less inhibitory effect on 3CLpro with IC50 value of 14.4μM.

CCF0058981 is a ML300-derived, non-covalent SARS-CoV‑2 3CLpro inhibitor.

Saquinavir is an HIV Protease inhibitor used in antiretroviral therapy. Saquinavir could inhibit SARS-CoV-2 3CLpro main protease.

Rosmariquinone showed inhibitory effect on PL(pro) and 3CLpro with IC50 values of 30μM and 21.1μM, respectively.